The main purposes of this research were to investigate the shyness of implicit and explicit measures by double dissociation design. In pilot study, 10 shyness and non-shyness words were chosen respectively through evaluative procedures, which were for subsequent implicit association test (IAT) of shyness. In formal study, its aims to assess the explicit shyness, implicit shyness and shy behaviors, and explored the role of social desirability in implicit and explicit measures of the shyness. The results suggested that shyness IAT is unrelated to the explicit shyness measure and the social desirability scale. It means that IAT can avoid the being influenced by social desirability, and were affected the same way on Chinese and Western subjects. Moreover, that shyness IAT and explicit shyness are two different construct, and also, with regard to the shy behaviors, it show that the IAT of shyness effect is significantly correlated with unconscious and automatic shy behaviors (implicit behaviors) of the subjects (such as face adaptors), and explicit shyness assessment is significantly correlated with conscious and controlled shy behaviors (explicit behaviors, such as smiling). This results support the shyness constructs in explicit and implicit assessment are different.