The aim of this study is to investigate and establish a Work Value Assembly (WVA) scale for assessing Taiwanese college students. The scale was initially constructed using qualitative research methodology, conducting focus group interviews to collect 254 descriptive sentences related to work values. Following step-by-step editing and revision, the items were categorized into seven factors: “Prosocial,” “Interpersonal,” “Prestige,” “Comfort,” “Professional Growth,” “Self-actualization,” and “Autonomy.” A total of 629 college students participated in the pretest. Exploratory Factor Analysis (EFA) was utilized to eliminate unsuitable items. After screening, each scale factor included six items, the scale had a total of 42 reliable items. Next, another group of 592 college students participated in a formal test. Internal consistency reliability (Cronbach´s α) ranged from .84 to .91. In a retest two weeks later, test-retest reliability coefficients ranged from .67 to .83. In terms of validity, Confirmatory Factor Analysis (CFA) showed that goodness-of-fit indices all fell within an ideal range. Based on these results, the WVA scale showed good reliability and validity in measuring the work values of Taiwanese college students. Lastly, we discussed the results, made recommendations to school counselors, and offered suggestions for further research.