This study examined individual differences in ambivalence over emotional expression (AEE), including gender, personality traits, cultural self-construal, and emotion regulation strategies. A total of 348 undergraduate students completed a packet of questionnaires related to ambivalence over emotional expression, cultural self-construal, and emotion regulation strategies. Results:(1) There was gender difference in AEE, men are be significantly more ambivalent over expression than women. (2) After controlling for gender, neuroticism could significantly predict AEE, but extraversion could not. (3) After controlling for gender and personality traits, the interdependent self could significantly predict AEE, but independent self could not. (4) AEE was significantly predicted by the emotional regulation strategies of rumination, seeking support, avoidance, and distraction. Implications of these findings for future research in the area of emotion expression and health were discussed.