The purpose of this study is to design a gratitude program for undergraduate students and to investigate its immediate and long-term effects on gratitude, well-being and resilience. The program includes five dimensions: thanking others, thanking God, cherishing what you have, appreciating hardship, and appreciating the moment. Moreover, the study is a quasi-experimental design. A total of 67 students from Kaohsiung participated in this study. In the experimental group, 36 students went through the 12-hour gratitude program. The rest of the 31 students were in the control group and didn’t undergo any training. The pre- and post-test included four questionnaires: Inventory of Undergraduates’ Gratitude, The Gratitude Questionnaire-6, Mental Health Continuum­Short Form, and Inventory of Adolescent Resilience. The researcher also analyzed a feedback questionnaire. The results showed: (1) The gratitude program elevated students’ gratitude, based on the five dimensions of the Inventory of Undergraduates’ Gratitude, (2) The gratitude program elevated students’ well-being, based on the three dimensions of Mental Health Continuum­Short Form. (3) The gratitude program elevated students’ resilience, based on the four dimensions of Inventory of Adolescent Resilience. The feedback questionnaire displayed that the gratitude program met students’ learning needs. Some concrete suggestions on education, student guidance, and research are also provided.