This study developed a career calling scale for working adults in Taiwan. In the first stage, the participants were 30 working adults over the age of 20. Focus group interviews were conducted to collect the data, that the author used to establish the scale as a pilot scale. In the second stage, three experts were invited to assess content validity. Then, a pre-test of the pilot scale was conducted to obtain 151 questionnaires, and the data was analyzed and selected under item analysis and factor analysis methods to establish a formal scale. The Career Calling Scale (CCS) contains 20 items rated on a 4-point scale and consists of four subscales: Interests and Passions, Life Meaning, Altruism, and Transcendent Summons. Higher scores people in each subscale show strong inclination toward that aspect. In the third stage, the participants were 548 working adults between the ages of 16 and 58 (M = 35.59, SD = 11.48). Reliability analysis indicated that the internal consistency of the four subscales was between .87 and .92, and the internal consistency of the whole scale was .97. The comparison of background variables showed that the scores for each subscale for social workers and enterprising workers were significantly higher than the scores for realistic workers. Using Job Satisfaction, Self-Efficacy, and Work Stress as criteria, the test showed high criterion-related validity. Confirmatory factor analysis also showed a good model fit, which supported good construct validity. At the end, a discussion based on the results and suggestions for practice and future studies are provided.